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Optimization of Purification Process for Total Alkaloids from Fritillaria thunbergii by Macroporous Resin

Liwei Han

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Abstract

Objective:To optimize purification process of total alkaloids from Fritillaria thunbergii by macroporous resin.Method:Optimum macroporous resin and its purification process was selected by static and dynamic adsorption and desorption kinetics test.Result:Optimal purification process conditions were:optimal macroporous resin type HPD100,diameter-height ratio 1∶9,the concentration of sample solution 5 g·mL-1 with pH as 8.0,maximum sample volume 13 BV,flow rate of adsorption and elution 1 mL·min-1,washed by 8 BV water and 10 BV 80% ethanol,collected eluent of 80% ethanol.Under this optimized technology,the content of total alkaloids was more than 65% by UV.Conclusion:HPD100 type macroporous resin showed good comprehensive adsorption property for enrichment of total alkaloids from F.thunbergii,and suitable for separation and purification of it.

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What this paper is about

Objective:To optimize purification process of total alkaloids from Fritillaria thunbergii by macroporous resin.Method:Optimum macroporous resin and its purification process was selected by static and dynamic adsorption and desorption kinetics test.Result:Optimal purification process conditions were:optimal macroporous resin type HPD100,diameter-height ratio 1∶9,the concentration of sample solution 5 g·mL-1 with pH as 8.0,maximum sample volume 13 BV,flow rate of adsorption and elution 1 mL·min-1,washed by 8 BV water and 10 BV 80% ethanol,collected eluent of 80% ethanol.Under this optimized technology,the content of total alkaloids was more than 65% by UV.Conclusion:HPD100 type macroporous resin showed good comprehensive adsorption property for enrichment of total alkaloids from F.thunbergii,and suitable for separation and purification of it.

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Available abstract

Objective:To optimize purification process of total alkaloids from Fritillaria thunbergii by macroporous resin.Method:Optimum macroporous resin and its purification process was selected by static and dynamic adsorption and desorption kinetics test.Result:Optimal purification process conditions were:optimal macroporous resin type HPD100,diameter-height ratio 1∶9,the concentration of sample solution 5 g·mL-1 with pH as 8.0,maximum sample volume 13 BV,flow rate of adsorption and elution 1 mL·min-1,washed by 8 BV water and 10 BV 80% ethanol,collected eluent of 80% ethanol.Under this optimized technology,the content of total alkaloids was more than 65% by UV.Conclusion:HPD100 type macroporous resin showed good comprehensive adsorption property for enrichment of total alkaloids from F.thunbergii,and suitable for separation and purification of it.

Key concepts: Adsorption, Chromatography, Elution, Desorption, Chemistry, Ethanol, Volume (thermodynamics), Column chromatography

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