Purification Process of Total Alkaloids from Houttuynia cordata by Macroporous Resin

Objective:To optimize separation and purification technology of total alkaloids from Houttuynia cordata by macroporous resin.Method:With the content of berberine hydrochloride,pepper lactam and aristololactam B as indexes,separation and purification capacity of total alkaloids from H.cordata was compared with different types of macroporous resin and different technology conditions.Result:HPD-100 macroporous resin had the best separation and purification capacity for total alkaloids in following technology conditions: sample concentration was 20 g·L-1 with pH 3,flow rate was 0.5 mL·min-1,eluting reagent was ethanol solution of ammonia(ammonia concentration 0.5 mol·L-1,the volume fraction of ethanol 80%) with the amount of 4 BV,eluting velocity was 3.0 mL·min-1.Conclution: HPD-100 macroporous resin could be applied to purify alkaloids from H.cordata.