Optimization of the microwave-assisted extraction technique of Pine Needles polysaccharides with response surface methodology

The microwave-assisted extraction technology was used to extract the Pine Needle polysac-charides.The experiment factor and levels were firsts selected by one-factor test,then the method of response surface analysis with three and levels was adopted and the factors influencing the technological paraments were determined by means of regression analysis.The results shows that the optimum conditions with the microwave-assisted extraction technique of polysaccharides from Pine Needles were: the ratio of water to solid 28∶1,extraction temperature of 89 ℃ and extraction time of 13 min.The yield of the Pine Needle polysac-charides is 6.2795% with three times.