Optimization of the extraction of polysaccharides from pine needles.

Process of extracting polysaccharides from 3 kinds of pine needles has been studied.During the extracting process,the contents of polysaccharides of three pine needles were compared between sovents of water and ethanol.Five main factors including ratio of solid to liquid,extracting temperature,extracting time,pH value and extraction times were studied on the yield of pine needles polysaccharides.Using single factor analysis,the optimal processing conditions for water extraction of polysaccharides were obtained by orthogonal experiments.The results showed that the optimum parameters were:extraction time 3h at 100℃ with 1∶14 ratio of solid to liquid,and pH value 6,and the yield of polysaccharides of P.elliottii was 4.024%±0.017%.