Ultrasonic-assisted Extraction of Polysaccharides from Pine Needles

Using the ultrasonic-assisted extraction method,process of extracting polysaccharides from pine needles was studied.With water as extraction solvent,5 factors including extraction temperature,ultrasonic power,ultrasonic extraction time,ratio of solid to liquid and extraction times were studied for their effect on the yield of pine needles polysaccharides.On the base of the single factor experiments,the optimal processing conditions for the extraction of polysaccharides were obtained by orthogonal experiments.The results showed that the optimum parameters were: ultrasonic extraction time 30 min,extraction temperature 79 ℃,ratio of solid to liquid 1:30(g/mL),and ultrasonic power 400 W.When extracting 1 times under these conditions,the yield of pine needles polysaccharides was 4.15%.