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2008•China Practical MedicineRequires access

Determination of content Paeoniflorin in Xiaosiyanger granule by HPLC

Guo Wei-zhon

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Abstract

Objective To develop a method for paeoniflorin in Xiaosiyanger.Methods HPLC with ODS-C18(5 μm,4.6 mm×250 mm)column was used.The mobile phase of paeoniflorin was a mixture of Acetonitrile-0.1%H3PO4(16:84),with the flow rate of 1.0 ml/min and detection wavelength at 230 nm.Results Paeoniflorin(0.2-1.0 μg)showed fine linear relationship(r=0.999 8),the average recovery was 99.47%(n=5),RSD=1.06%.Conclusion The method is quick,simple and accurate and can be the method to determination of content Paeoniflorin in Xiaosiyanger granule.

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What this paper is about

Objective To develop a method for paeoniflorin in Xiaosiyanger.Methods HPLC with ODS-C18(5 μm,4.6 mm×250 mm)column was used.The mobile phase of paeoniflorin was a mixture of Acetonitrile-0.1%H3PO4(16:84),with the flow rate of 1.0 ml/min and detection wavelength at 230 nm.Results Paeoniflorin(0.2-1.0 μg)showed fine linear relationship(r=0.999 8),the average recovery was 99.47%(n=5),RSD=1.06%.Conclusion The method is quick,simple and accurate and can be the method to determination of content Paeoniflorin in Xiaosiyanger granule.

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Available abstract

Objective To develop a method for paeoniflorin in Xiaosiyanger.Methods HPLC with ODS-C18(5 μm,4.6 mm×250 mm)column was used.The mobile phase of paeoniflorin was a mixture of Acetonitrile-0.1%H3PO4(16:84),with the flow rate of 1.0 ml/min and detection wavelength at 230 nm.Results Paeoniflorin(0.2-1.0 μg)showed fine linear relationship(r=0.999 8),the average recovery was 99.47%(n=5),RSD=1.06%.Conclusion The method is quick,simple and accurate and can be the method to determination of content Paeoniflorin in Xiaosiyanger granule.

Key concepts: Paeoniflorin, Medicine, Granule (geology), Chromatography, High-performance liquid chromatography, Content determination, Linear relationship, Chemistry

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