Content determination of paeoniflorin in Jianweixiaoyan Granule by HPLC
Jian‐Xia Jiang
Abstract
Jian‐Xia Jiang
Abstract
Objective:To establish a method for the content determination of paeoniflorin in Jianweixiaoyan Granule by HPLC.Methods:The luna-C18 (4.6 mm×150 mm,5 μm) column was used.The mobile phases consisted of acetonitrile and water (13:87),the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm.Results:The linear ranges of paeoniflorin was at 0.100 8-2.016 0 μg (r=1.000 0),the average recovery was 100.59% with a RSD of 0.75% (n=6).Conclusion:The method is fast,reliable,accurate and can be used in the quality control of paeoniflorin in Jianweixiaoyan Granule.
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Objective:To establish a method for the content determination of paeoniflorin in Jianweixiaoyan Granule by HPLC.Methods:The luna-C18 (4.6 mm×150 mm,5 μm) column was used.The mobile phases consisted of acetonitrile and water (13:87),the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm.Results:The linear ranges of paeoniflorin was at 0.100 8-2.016 0 μg (r=1.000 0),the average recovery was 100.59% with a RSD of 0.75% (n=6).Conclusion:The method is fast,reliable,accurate and can be used in the quality control of paeoniflorin in Jianweixiaoyan Granule.
Key concepts: Paeoniflorin, Medicine, Granule (geology), Chromatography, High-performance liquid chromatography, Content determination, Linear relationship, Composite material