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2003•Yiyao daobaoRequires access

Determination of Paeoniflorin in Gongyankang Granule by HPLC

Xiong Wen-bi

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Abstract

Objective:To establish a HPLC method for determination of paeoniflorin in gongyankang granule. Method:The content of paeoniflorin was determined by HPLC, using VYDAC218TP54 column; acetonitrile-0.1% phosphoric acid(15∶85) as mobile phase; The detective wavelength was 243 nm. Results:Paeoniflorin at the concentrations of 0.098 2-0.785 6 μg showed good linearity ( r =0.999 7). The average recovery was 103.7% with RSD 2.6%. Conclusion:The results show that the method is simple and accurate. It can be useful in quality control of the products.

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What this paper is about

Objective:To establish a HPLC method for determination of paeoniflorin in gongyankang granule. Method:The content of paeoniflorin was determined by HPLC, using VYDAC218TP54 column; acetonitrile-0.1% phosphoric acid(15∶85) as mobile phase; The detective wavelength was 243 nm. Results:Paeoniflorin at the concentrations of 0.098 2-0.785 6 μg showed good linearity ( r =0.999 7). The average recovery was 103.7% with RSD 2.6%. Conclusion:The results show that the method is simple and accurate. It can be useful in quality control of the products.

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Available abstract

Objective:To establish a HPLC method for determination of paeoniflorin in gongyankang granule. Method:The content of paeoniflorin was determined by HPLC, using VYDAC218TP54 column; acetonitrile-0.1% phosphoric acid(15∶85) as mobile phase; The detective wavelength was 243 nm. Results:Paeoniflorin at the concentrations of 0.098 2-0.785 6 μg showed good linearity ( r =0.999 7). The average recovery was 103.7% with RSD 2.6%. Conclusion:The results show that the method is simple and accurate. It can be useful in quality control of the products.

Key concepts: Paeoniflorin, Chromatography, Granule (geology), High-performance liquid chromatography, Phosphoric acid, Chemistry, Acetonitrile, Materials science

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