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2004•Strait Pharmaceutical JournalRequires access

Determination of Paeoniflorin in Kuntaian Granule by HPLC

Miao Jian-quan

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Abstract

OBJECTIVE To establish a method for the determination of paeoniflorin in Kuntaian Granule.METHODS The sample was extracted with methyl.The chromatographic conditions were as follow:Zorbax SB-C 18 (250mm×4.6mm,5μm)with a mobil phase of acetonitrile-0.01% phosphoric acid(15∶85),the detection wavelength at 230nm and the flow rate being 1.0ml·min -1 .RESULTS A linearity was obtained from 0.308μg to 3.696μg of paeoniflorin with a good correlation(r=0.99998,n=6).The average recovery was 98.50% and RSD was 0.74%(n=5).CONCLUSION The results showed that this method for determination of paeoniflorin in Kuntaian Granule is easy,sensitive,specific and accurate.

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OBJECTIVE To establish a method for the determination of paeoniflorin in Kuntaian Granule.METHODS The sample was extracted with methyl.The chromatographic conditions were as follow:Zorbax SB-C 18 (250mm×4.6mm,5μm)with a mobil phase of acetonitrile-0.01% phosphoric acid(15∶85),the detection wavelength at 230nm and the flow rate being 1.0ml·min -1 .RESULTS A linearity was obtained from 0.308μg to 3.696μg of paeoniflorin with a good correlation(r=0.99998,n=6).The average recovery was 98.50% and RSD was 0.74%(n=5).CONCLUSION The results showed that this method for determination of paeoniflorin in Kuntaian Granule is easy,sensitive,specific and accurate.

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Available abstract

OBJECTIVE To establish a method for the determination of paeoniflorin in Kuntaian Granule.METHODS The sample was extracted with methyl.The chromatographic conditions were as follow:Zorbax SB-C 18 (250mm×4.6mm,5μm)with a mobil phase of acetonitrile-0.01% phosphoric acid(15∶85),the detection wavelength at 230nm and the flow rate being 1.0ml·min -1 .RESULTS A linearity was obtained from 0.308μg to 3.696μg of paeoniflorin with a good correlation(r=0.99998,n=6).The average recovery was 98.50% and RSD was 0.74%(n=5).CONCLUSION The results showed that this method for determination of paeoniflorin in Kuntaian Granule is easy,sensitive,specific and accurate.

Key concepts: Paeoniflorin, Granule (geology), Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Linear correlation, Materials science

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