Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.

The fasciculated shoots were induced by the explant achieved from the juvenile branches of Laurocerasus caroliniana Ait.in spring.The inducing rate of fasciculated shoots attained 100% when they were cultured on the inducing medium of MS with BA 0.5 mg/L and NAA 0.01 mg/L.The adventitious shoots were propagated most rapidly when subcultured on the medium of MS with BA 1.0 mg/L and NAA 0.1 mg/L.Moreover,the adventitious shoots with best quality were obtained when they were cultured in the medium of MS with KT 1.0 mg/L and NAA 0.1 mg/L.The results of this experiment also indicated that gibberellin had no effects on the elongation of adventitious shoots.When the 3—4 cm adventitious shoots were cut and rooted in the rooting medium of 1/2 MS with NAA 1.0 mg/L,the rooting rate and surviving rate attained 100% and 40% respectively.