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Establishment of Rapid Micropropagation System of Ligustrum lucidum by Tissue Culture

Shuyu Li

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Abstract

Take one-year-old shoots,buds of new shoots,current-year shoots,sprouting shoots and autumn shoots as explants in tissue culture.The results showed that the best disinfectant was 0.1%HgCl_2 with 5min treatment.The best explant was the current-year shoots and the sprouting shoots.The best culture medium of the sprouting shoots was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best culture medium of the current-year shoots was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L.The best culture medium of clump shoots was MS+6-BA 4.0 mg/L+NAA 0.1 mg/L.

About this research paper

What this paper is about

Take one-year-old shoots,buds of new shoots,current-year shoots,sprouting shoots and autumn shoots as explants in tissue culture.The results showed that the best disinfectant was 0.1%HgCl_2 with 5min treatment.The best explant was the current-year shoots and the sprouting shoots.The best culture medium of the sprouting shoots was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best culture medium of the current-year shoots was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L.The best culture medium of clump shoots was MS+6-BA 4.0 mg/L+NAA 0.1 mg/L.

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Available abstract

Take one-year-old shoots,buds of new shoots,current-year shoots,sprouting shoots and autumn shoots as explants in tissue culture.The results showed that the best disinfectant was 0.1%HgCl_2 with 5min treatment.The best explant was the current-year shoots and the sprouting shoots.The best culture medium of the sprouting shoots was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best culture medium of the current-year shoots was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L.The best culture medium of clump shoots was MS+6-BA 4.0 mg/L+NAA 0.1 mg/L.

Key concepts: Shoot, Sprouting, Explant culture, Micropropagation, Tissue culture, Botany, Biology, Murashige and Skoog medium

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