Tissue Culture and Plant Regeneration of Cerasus campanulata

Stabilized shoot cultures were achieved when the explant sources were the juvenile branches of Cerasus campanulata in spring.The inducing rate of fasciculated shoots was 87.5% when the inducing medium of 1/4MS with BA 1.0 mg/L and IBA 0.01 mg/L were used.The adventitious shoots were propagated most rapidly when subcultured on the medium of MS with BA 1.0 mg/L,IBA 0.1 mg/L and GA 3 0.3 mg/L,or MS with KT1.0 mg/L,NAA 0.1 mg/L and GA 3 0.3 mg/L.The elongation of adventitious shoots was greatly affected by gibberellin.The adventitious shoots were cut and rooted in the rooting medium of 1/2MS with NAA 1.0 mg/L IBA 1.0 mg/L and BA 0.75 mg/L.The tooting rate was more than 90%.The surviving rate was 95% when the intact shoots were moved to the greenhouse.