Study on Regeneration Plant of Prunus Wilsonil Leaves in vitro

Rapid propagation of Prunus Wilsonil was preliminary stuidied using leaves of testtube seedling for in vitro culture.The results showed that the a chip of leaf from test-tube plantlet were cultured,a mass of adventitious shoots were induced with MS medium containing BA 0.5 mg/L + NAA 0.01 mg/L,its induction rate was 50.1%;and combination with BA 0.5 mg/L + NAA 0.5 mg/L + KT 0.5 mg/L + IBA 0.2 mg/L,adventitious shoots could be used repeatedly to subculture proliferation:rooting was induced with adventitious shoots in MS supplemented of IBA 0.4 mg/L,rooting rate was 67%.Thus the leaf of testtube seedling can be used as explant for rapid propagation of Prunus Wilsonil.