Effects of 4-PU and 6-BA on Formation of Calli and Adventitious Buds in Epicotyl Explants of Arachis hypogaea

The effects of 4-PU and 6-BA on formations of the calli and adventitious buds from epicotyl explants in peanut were studied in the experiment. Cultured in the MS medium contained 0.1mg/L 4-PU and 6-BA 4mg/L for 20 days, the formation rate of calli could reach 100%. 0.1mg/L 4-PU combined with 1-4mg/L 6-BA in MS medium promoted the adventitious buds formation. The optimal medium for adventitious bud formation and shoot elongation were MS +0.1 mg/L 4-PU + 2mg/L 6-BA and MS+0.1mg/L 4-PU+4mg/L BA, respectively. The percentage of adventitious bud formation, number and length of buds of peanut grown in the MS medium contained only 0.5mg/L 4-PU were higher than those grown in the MS medium contained 0.5mg/L 4-PU+6-BA.