In vitro Culture of Radish Tissues and Organs

The induction,proliferation and re-differentiation of adventitious buds and calli of radish cultivar 'LU-Radish-NO.1'were explored in this experiment.Results showed that the stem tip with hypocotyl was the optimal explant for adventitious buds induction.MS+3 mg/L 6-BA+0.2 mg/L NAA was the best medium for adventitious buds proliferation.However,when the buds were subcultured for 5 generations on this medium,the concentration of 6-BA should be promoted properly to keep the higher proliferation level.The medium MS+3 mg/L 6-BA+0.3 mg/L NAA with cytoledons as explants was better for calli induction.When 5 mg/L AgNO3 was added,the medium was benefit for calli subculture and preservation.The regenerated buds had been obtained when the induced calli were transferred to the medium MS+6 mg/L 6-BA+0.1 mg/L NAA+5 mg/L AgNO3.