The Restrictive Factors for Induction from Various Peanut Explant

The restrictive factors for adventitious bud induction from leaflet were investigated using the 12d and 4d seedlings of peanut variety Yuanza 9102. Results showed that the adventitious bud induction rate reached to 70% in the MS added with high concentrations of 6-BA(8mg/L), low concentrations of NAA(1mg/L), indicating that the MS medium+8mg/L 6-BA+0.5mg/L NAA+2mg/L AgNO3 had the best result for inducing the adventitious buds. The best medium for subculture was MS+5mg/L 6-BA+2mg/L NAA+2mg/L AgNO3. And the adventitious bud induction rate of 4d seedlings of peanut was higher than the 12d. It was also observed that the underside cut near the petiole presented a higher rate of adventitious buds, so was the ideal explant for adventitious bud induction. The tissue culture of peanut was studied using 2d cotyledon explant of peanut variety Yuanza 9102. The result showed that the best medium for the induction of callus was MS+6-BA4.5mg/L+2,4-D 2.2mg/L, The callus induction rate reached to 79.8%. And MS+KT (0.15mg/L) was the best differentiation medium.