The Restrictive Factors for Efficient Induction of Adventitious Buds from Peanut Leaflet in vitro

The efficiency of adventitious bud induction from leaflet were investigated using the 4-day seedlings of peanut variety Yuhua 14.The MS medium was added with commonly-used kinin 6-BA,kinetin NAA,and uncommonly-used ABA and thiadiazole Diazo phenyl urea TDZ.Results showed that the adventitious bud induction rate was low in the MS added with only 6-BA,NAA and TDZ,but reached to 80% in the MS added with high concentrations of 6-BA(8mg/L),low concentrations of NAA(1mg/L) and low concentrations ABA(1mg/L),indicating that the MS medium+8mg/L 6-BA+1mg/L NAA+1mg/L ABA+2mg/L AgNO3 had the best result for inducing the adventitious buds.The best medium for subculture was MS+5mg/L 6-BA+2mg/L NAA+2mg/L AgNO3.It was also observed that the underside cut near the petiole presented a higher rate of adventitious buds,so was the ideological explant for adventitious bud induction.