An experimental study of insulin and IGF-I on promoting osteoblast proliferation

Objective:To investigate the effect of insulin(INS)and insulin like growth factor-Ⅰ(IGF-Ⅰ)on proliferation of the human osteosarcoma cell line MG63(MG63) in vitro.17-?茁 estradiol(E2)were used as the contrast medicine.This experiment focus on the exploration of the pathogenesis of the osteoporosis(OP) and the providing of the experimental basis for treatment of OP.Methods:The MG63 cells(105/ml)were seeded into the phenol-free DMEM medium with 10% fetals bovine serum(FBS)for 24 hours.Then the cultured MG63 were exposed to INS,IGF-Ⅰ and E2 of five different concentrations(10 paralleled wells for each concentration of every medicine)and then were cultured 44 hours with 0.1% bovine serum albumin(BSA)phenol-free DMEM.Zero concentration served as control group(CON).After the 3-[4,5-dimethythiazolzyl]-2,5-diphenyl tetrazo-diumbromide(MTT) staining,the optical density(OD)numbers of INS,IGF-Ⅰ and E2 groups were examined.Results:The OD numbers of INS,IGF-Ⅰ and E2 groups were significantly different to CON (P0.05).The effect of three medicines on MG63 was dose-dependently increased at the best effective concentration.The MG63 proliferation rates of three medical groups had markedly difference(P0.05)and the best medical effect concentrations of the three medicine are 50nM,10nM and 100nM respectively.Conclusion:Like the function of E2,INS and IGF-Ⅰ promote the proliferation of MG63.