Methylene blue staining examination for the proliferation of human osteosarcoma cell line MG63

?Objective To investigate the effect of insulin(INSU)and insulin like growth factor-1(IGF-1) on proliferation of the human osteosarcoma cell line MG63(MG63) in vitro.and to observe the changes of cell proliferation thus to provide evidence for OP treatment.Methods The MG63 cells(10~5/mL) were transferred into the phenol-free DMEM medium with 10% fetals bovine serum (FBS) for 24 hours.The cultured MG63 were exposed to five different concentrations of INSU,IGF-1 and E_2 (10 paralleled wells for every each concentration of medicine) followed by 44 hours culture with 0.1% bovine serum albumin(BSA) phenol-free DMEM. Zero concentration served as control group (CON).17-β estriadiol (E_2) was used as positive control.After stained by 3-[4,5-Dimethythiazolzyl]-2,5-Diphenyl Tetrazo-diumbromide,the OD of INSU,IGF-1 and E_2 groups were examined.Results No difference was found in the proliferation rate among the three groups(P0.05),but significant difference was shown within each group between pre-and after medication was added(P0.05).The changes in proliferation rate was dose-independent.Conclusions INSU and IGF-1 can promote MG63 proliferation as E_2.