Effect of insulin and IGF-1 on expression of typeIcollagen of human osteosarcoma cell line MG 63

Objective To investigate the effect of insulin (INSU) and insulin like growth factor-Ⅰ (IGF-Ⅰ) on the expression of typeⅠcollagen(COL1) with positive of 17-β estradiol(E_2) and to explore the pathogenesis of the osteoporosis. Methods MG 63 cells(10~5/mL) were cultured in the DMEM medium and exposed to INSU,IGF-1 or E_2 for 48 hours with 0.1% bovine serum albumin phenol-free DMEM. The total RNA of each sample was extracted and reverse transcriptions was performed to get the cDNA chains of COL1. Fluorescence real time quantitative PCR assay was carried out to examine the mRNA expression of COL1. The nest primers were used in the PCR assay. ANOVA and post hoc analysis were performed in SPSS 11.0 software. Results The expression of mRNA for COL1 was significantly different among the three groups (P0.05) and the effect of each medication increased dose-dependently. The most significant effect was observed at the highest concentration of each medication applied in this study (INSU 2×10~ -7 mol/L, IGF-1 10~ -7 mol/L and 17-β estradiol 10~ -6 mol/L). The increase rates of COL1 mRNA in INSU, IGF-1 group was significantly higher than that of E_2 group (P0.05), but not between the groups of INSU and IGF-1 (P0.05). Conclusions INSU and IGF-1 can improved the differentiation of MG 63 as E_2.