Effects of IGF-Iand TGF-β1 on proliferation and differentiation of human osteoblast-like cell

【Objective】To investigate the effects of single and combined use of insulin-like growth factgor Ⅰ(IGF-Ⅰ) and transforming growth factor β1(TGF-β1) on the proliferation and differentiation of human osteoblastlike cell MG-63 cultured in vitro.【Methods】①MG-63 cells cultured in vitro were treated with different dosages of rhIGF-Ⅰ(0.1,1.0,10.0 and 100.0 ng/mL) or rhTGF-β1(0.01,0.1,1.0,10.0 ng/mL). Cell proliferation was measured by methylthiazol tetrazolium(MTT) colorimetric method to choose an optimal dosage of rhIGF-Ⅰ and rhTGFβ1. ②MTT method and alkaline phosphatase(ALP) measurement kit were used to determine the proliferation at different time points(1,3,5 and 7 days) and ALP activity of MG-63 cell after optimal dosage of rhIGF-Ⅰor/and rhTGF-β1 were added into the culture medium.【Results】①Compared with control group,both IGF-Ⅰ and TGF-β1 significantly promoted the proliferation of MG-63(P 0.05). The cell number in osteoblasts exposed to IGF-Ⅰtreated cells was higher than that in TGF-β1-treated cells(P 0.05). ②When using different dosages,10 ng/mL IGF-Ⅰ and 1ng/mL TGF-β1 showed the strongest effects of promoting proliferation(P 0.05). ③The combined group of IGF-Ⅰ(10 ng/mL) and TGF-β1(1 ng/mL) showed an even higher proliferative effect than using them respectively. ④Compared with control group,significant changes of cellular ALP activities were found when using IGF-Ⅰ or TGF-β1 while combining them showed stronger effects.【Conclusion】The combined use of IGF-Ⅰand TGF-β1 in a proper concentration significantly enhances the proliferation and differentiation of human osteoblasts cultured in vitro.