Proliferation inhibition and apoptosis induction in human endometrial carcinoma cell line HEC-1-B by diallyl disulfide

Objective To investigate the impact of DADS on the proliferation inhibition and apoptosis induction in human endometrial carcinoma cell line HEC-1-B.Methods MTT assay was used to detect the proliferation inhition of DADS on HEC-1-B cells.AnnexinV/PI staining was performed to quantify apoptotic cells by flow cytometry.Hoechst-33528 stanining was employed to observe the apoptosis morphology.Results After DADS treatment at the concentration of 100,200,400 μmol/L for 24,48 h,the proliferation could be inhibited at the concentration of 100 μmol/L.The growth inhibition of DADS was strengthened in a dose-time-dependent manner.After DADS treatment for 48 h at the concentration of 200 μmol/L,the ratio of apoptosis cells was 40.91% by fluorescence microscopy and flow cytometry.Obvious apoptotic morphology changes was observed by Hoechst-33528 stanining.Conclusion DADS can induce proliferation inhibition and apoptosis in HEC-1-B cells,which indicates that DADS may be therapeutically useful to endometrial carcinoma chemotherapy.