Effect of Diallyl Disulfide on the Proliferation,Cell Cycle,Apoptosis of Human Leukemia Cell Line K562

Objective: To explore the mechanism of cell proliferation-inhabit and apotosis of human leukemia cell line K562 treated with diallyl disulfide(DADS).Methods: MTT assay,flow cytometry and immunohistochemistry were employed to examine DADS-induced apoptosis and growth inhibition in human Leukemia cell Line K562.Rusults:1.DADS inhibited proliferation of K562 cells from 10～80mg.L-DADS in a dose-and time-dependent manner;2.K562 cells treated with DADS after the treatment for 24 hours significantly increased in the percentage of cells in the G2/M phase;3.From 10～80mg.L-DADS induced apotosis of K562 cells after the treatment for 24 hours,the difference is significant;4.DADS could up-regulate the level of protein of p21WAF1 in K562 cells.Conclusions:DADS has apparent effects of proliferation-inhabit and apoptosis on K562 cells in vitro,its anti-leukemia mechanisms may be related to up-regulate the gene of of p21WAF1 in K562 cells.