Growth Inhibition and Apoptosis of K562 Cells Induced by Diallyl Disulfide

Objective Diallyl Disulfide(DADS) is a natural compound derived from crushed garlic.This paper is to explore the proliferation inhibited,apoptosis induced and cell cycle regulated effect of DADS on human K562 cells.Methods The CCK-8 assay,morphological analysis,DNA fragmentation technique and flow cytometry analysis were used.Results After 48 hours of treatment with(15,30,60,120,240 μmol/L) DADS,the inhibitory rate of human K562 cells was 26.3%,58.2%,44.7%,62.6%,75.6%(P0.05) ,respectively.Meanwhile the K562 cells showed typical apoptosis changes,a typical sub-peak before G0/G1 was observed by flow cytometry and cell cycle was arrested in the G2/M phase,after 12 hours treatment with DADS at the different concentration(30,60,120 μmol/L) ,the rate of apoptosis of K562 cells was 18.59%±0.81%,20.85%±0.65%,23.5%±1.1%,respectively.Conclusion The DADS-treated K562 cells showed a dose-dependent decrease in cell viability and proliferation,and its possible mechanism was involved in apoptosis and G2/M arrest induced by DADS.