Effect of diallyl disulfide on the growth inhibition and apoptosis in human HepG_2 cancer cell line in vitro

Objective:To investigate the effect of diallyl disulfide(DADS)on the growth inhibition and apoptosis in human HepG2 cancer cell line in vitro.Methods:Effect of DADS on HepG2 cancer cell line proliferation was measured by MTT assay and cell counts.The apoptosis rate and phase distribution of cell cycle of cell line induced by DADS was detected by using flow cytometry(FCM) with PI staining.Results:DADS obviously inhibited cells proliferation,and the inhibition rate assumes the dependence of the drug concentration and time(P0.05);cell doubling time in DADS group extended(P0.05).Compared with the control group,G2 /M phase cell fraction increased significantly in the DADS treatment group,while G0 /G1 phase cell fraction decreased obviously, and the cell apoptosis rate increased significantly(P0.05).Conclusions:DADS could significantly inhibit the proliferation of human HepG2 cancer cell line and this effect presents dose-dependent and time-dependent.Besides,it can inhibit the cell cycle in G2 /M phase and induce apoptosis.