Pharmacokinetics of curdione and germacrone in zedoary turmeric oil in rats in vivo

AIM To study pharmacokinetic characteristics of curdione and germacrone in zedoary turmeric oil( ZTO) in rats. METHODS ZTO was extracted by vapour distillation,rat plasma samples were collected after oral administration. The chromatographic separation was achieved with a linear gradient elution of acetonitrile in water with a flow rate at 1. 0 mL / min on a Hypersil ODS- 2 column( 250 mm × 4. 6 mm,5 μm). Detection was carried out using a UV detector at 214 nm. RESULTS Curdione and germacrone in plasma samples had no interference. The linear range for curdione and germacrone were 1. 073 ~ 68. 64 μg / mL and 0. 1972 ~ 12. 62 μg / mL,respectively. Precision,stability and recoveries were in line with the requirements of methodology in vivo. CONCLUSION The developed method can be applied to the determination of curdione and germacrone in rats plasma. Pharmacokinetic study reveals that distribution of curdione and germacrone in ZTO is fit in with two-compartment model.