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Determination of Four Constituents in Oleum Curcumae And Zedoary Turmeric Oil Injection by HPLC

Yuan Wen

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Abstract

OBJECTIVE To establish a HPLC method for the determination of three constituents curdione,curcumol and germacrone in zedoary turmeric oil injection.METHODS The sample was analyzed on SHIMADZU VP-ODS C18(4.6 mm×250 mm,5 μm)column with mobile phase consisting of 0.1% phosphoric acid and acetonitrile by gradient eluteion at the flow rate of 1.0 mL·min-1.The wavelength was at 210 nm.RESULTS The good linearity,recovery and reproducibility were obtained.The mean recoveries were above 99.0% and RSDs were below 20%.CONCLUSION The method is simple,quick to process and accurate,and can be used for quality control for zedoary turmeric oil injection.

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What this paper is about

OBJECTIVE To establish a HPLC method for the determination of three constituents curdione,curcumol and germacrone in zedoary turmeric oil injection.METHODS The sample was analyzed on SHIMADZU VP-ODS C18(4.6 mm×250 mm,5 μm)column with mobile phase consisting of 0.1% phosphoric acid and acetonitrile by gradient eluteion at the flow rate of 1.0 mL·min-1.The wavelength was at 210 nm.RESULTS The good linearity,recovery and reproducibility were obtained.The mean recoveries were above 99.0% and RSDs were below 20%.CONCLUSION The method is simple,quick to process and accurate,and can be used for quality control for zedoary turmeric oil injection.

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Available abstract

OBJECTIVE To establish a HPLC method for the determination of three constituents curdione,curcumol and germacrone in zedoary turmeric oil injection.METHODS The sample was analyzed on SHIMADZU VP-ODS C18(4.6 mm×250 mm,5 μm)column with mobile phase consisting of 0.1% phosphoric acid and acetonitrile by gradient eluteion at the flow rate of 1.0 mL·min-1.The wavelength was at 210 nm.RESULTS The good linearity,recovery and reproducibility were obtained.The mean recoveries were above 99.0% and RSDs were below 20%.CONCLUSION The method is simple,quick to process and accurate,and can be used for quality control for zedoary turmeric oil injection.

Key concepts: Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Oleum, Reproducibility, Organic chemistry

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