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Determination of the Content of Paeoniflorin in Jiangchi Gel by HPLC

Wang Zhiyong

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Abstract

Objective To establish a method for determinating paeoniflorin in jiangchi gel by HPLC. Methods The chromatographic conditions were as follows: CLC-ODS column(5 μm,150 mm×60 mm)used as the stationary phase,and methanol-water(45:55) as mobile phase with a flow rate of 1.0 mL·min-1,and the detecting wavelength 230 nm. Results A good linearity was obtained within the range of 0.08～0.80 μg·mL-1 of paeoniflorin(r=0.999 6).The average recovery rate of paeoniflorin was 100.61%(n=6,RSD=0.89%). Conclusion This method is simple,rapid and accurate which can be used to determine paeoniflorin contents in jiangchi gel.

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Objective To establish a method for determinating paeoniflorin in jiangchi gel by HPLC. Methods The chromatographic conditions were as follows: CLC-ODS column(5 μm,150 mm×60 mm)used as the stationary phase,and methanol-water(45:55) as mobile phase with a flow rate of 1.0 mL·min-1,and the detecting wavelength 230 nm. Results A good linearity was obtained within the range of 0.08～0.80 μg·mL-1 of paeoniflorin(r=0.999 6).The average recovery rate of paeoniflorin was 100.61%(n=6,RSD=0.89%). Conclusion This method is simple,rapid and accurate which can be used to determine paeoniflorin contents in jiangchi gel.

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Available abstract

Objective To establish a method for determinating paeoniflorin in jiangchi gel by HPLC. Methods The chromatographic conditions were as follows: CLC-ODS column(5 μm,150 mm×60 mm)used as the stationary phase,and methanol-water(45:55) as mobile phase with a flow rate of 1.0 mL·min-1,and the detecting wavelength 230 nm. Results A good linearity was obtained within the range of 0.08～0.80 μg·mL-1 of paeoniflorin(r=0.999 6).The average recovery rate of paeoniflorin was 100.61%(n=6,RSD=0.89%). Conclusion This method is simple,rapid and accurate which can be used to determine paeoniflorin contents in jiangchi gel.

Key concepts: Paeoniflorin, Chromatography, Chemistry, High-performance liquid chromatography, Stationary phase, Content determination, Methanol, Organic chemistry

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