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2007•Qilu Pharmaceutical AffairsRequires access

Determination of Paeoniflorin in Penyanxiao Gel by HPLC

YU Wei-ping

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Abstract

OBJECTIVE To determine Paeoniflorin in Penyanxiao Gel.METHODS The chromatographic conditions were as follow:Nucleosil C18 column,a mixture of methanol-0.05 mol·L-1 NaH2PO4(30∶70) as the mobile phase and the detection wavelength at 230 nm.RESULTS The calibration curves were linear in the range of 26.4～237.6μg·mL-1 for Paeoniflorin.The average recovery of Paeoniflorin were 99.27%.CONCLUSION This method was accurate,simple and convenient,rapid and reproducible,can be used for the quality control of Penyanxiao Gel.

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What this paper is about

OBJECTIVE To determine Paeoniflorin in Penyanxiao Gel.METHODS The chromatographic conditions were as follow:Nucleosil C18 column,a mixture of methanol-0.05 mol·L-1 NaH2PO4(30∶70) as the mobile phase and the detection wavelength at 230 nm.RESULTS The calibration curves were linear in the range of 26.4～237.6μg·mL-1 for Paeoniflorin.The average recovery of Paeoniflorin were 99.27%.CONCLUSION This method was accurate,simple and convenient,rapid and reproducible,can be used for the quality control of Penyanxiao Gel.

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Available abstract

OBJECTIVE To determine Paeoniflorin in Penyanxiao Gel.METHODS The chromatographic conditions were as follow:Nucleosil C18 column,a mixture of methanol-0.05 mol·L-1 NaH2PO4(30∶70) as the mobile phase and the detection wavelength at 230 nm.RESULTS The calibration curves were linear in the range of 26.4～237.6μg·mL-1 for Paeoniflorin.The average recovery of Paeoniflorin were 99.27%.CONCLUSION This method was accurate,simple and convenient,rapid and reproducible,can be used for the quality control of Penyanxiao Gel.

Key concepts: Paeoniflorin, Chromatography, Calibration curve, Chemistry, High-performance liquid chromatography, Methanol, Detection limit, Organic chemistry

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