Study On In Vitro Maturation Of Porcine Oocytes and Ethanol Activation

The objective of the present study was to investigate the effect of two different basic in vitro maturation(IVM) mediums(mTCM-199、NCSU23),concentration and treatment time of pregnant mare serum gonadotropin(PMSG) and human chorionic gonadotropin(HCG) on IVM of porcine oocytes;furthermore,to examine the effect of ethanol and ethanol plus various chemical agents on parthenogenetic activation and the development of parthenogenetic embryos. The rational IVM maturation of porcine oocytes system is NCSU23+20IU/mlPMSG+20IU/mlHCG+10%PFF,of which the maturation rate was 70.3+3.9%.The best parthenogenetic activation concentration of ethanol was 8%( activation rate : 29.6+2.5%).The best activation length of exposure by 8% ethanol was 10 min(activation rate : 32.6+4.1% ). Activation by ethanol associated with CB+CHX got the highest activation rate(60.8+3.2%),and the rate of cleavage,3~4cells,8cells and more than 8cells were 45.1+3.0%, 28.6+3.7%, 8.7+1.2%,respectively.Treated by ethanol associated with CB+ 6-DMAP,the rates of activation, cleavage, 3~4 cells, 8cells and more than 8cells were 51.7+1.9%, 41.7+3.6%, 30.3+4.3%, 9.9+1.8%, respectively; although the rates of activation and cleavage were lower than that of ethanol plus CB+CHX, the rates of 3~4 cells, 8cells and more than 8cells were higher. These results indicate that the best method of activation of pig oocytes by ethanol plus various chemical agents was 8% ethanol treating for 10 min,and then oocytes treated by CB+6-DMAP for another 7 h.