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2005•Zhongguo shouyi xuebaoRequires access

Effects of ethanol activation on the rates of cleavage and blastocyst of porcine IVM oocytes

Jianhui Tian, Shien Zhu, Zeng SengMing, Guoshi Liu, Yuan Chai, Liu Huan-ying, Lishmanova Ng, Zhongcheng Zhang

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Abstract

Effects of ethanol and combined with cytochalasin B(CB) or cycloheximide(CHX) or 6-dimethylaminopurine(DMAP) on the activation of porcine oocytes at 44 h of maturation in vitro were investigated.In this experiment,the development rates of ethanol-induced parthenogenetic activation of oocytes were compared.No blastocysts developed when ethanol alone at various concentrations(5%-11%) were used for 2 to 10 minutes.For the combination methods,the activation rate of oocytes was (70.86±3.75)% when exposed to 8% of ethanol for 5 min and treated in activation medium containing CB and DMAP for 6 h,and (15.07±5.53)% of parthenotes developed to blastocyst stage,higher(P0.05) than the other combined treatments.

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What this paper is about

Effects of ethanol and combined with cytochalasin B(CB) or cycloheximide(CHX) or 6-dimethylaminopurine(DMAP) on the activation of porcine oocytes at 44 h of maturation in vitro were investigated.In this experiment,the development rates of ethanol-induced parthenogenetic activation of oocytes were compared.No blastocysts developed when ethanol alone at various concentrations(5%-11%) were used for 2 to 10 minutes.For the combination methods,the activation rate of oocytes was (70.86±3.75)% when exposed to 8% of ethanol for 5 min and treated in activation medium containing CB and DMAP for 6 h,and (15.07±5.53)% of parthenotes developed to blastocyst stage,higher(P0.05) than the other combined treatments.

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Available abstract

Effects of ethanol and combined with cytochalasin B(CB) or cycloheximide(CHX) or 6-dimethylaminopurine(DMAP) on the activation of porcine oocytes at 44 h of maturation in vitro were investigated.In this experiment,the development rates of ethanol-induced parthenogenetic activation of oocytes were compared.No blastocysts developed when ethanol alone at various concentrations(5%-11%) were used for 2 to 10 minutes.For the combination methods,the activation rate of oocytes was (70.86±3.75)% when exposed to 8% of ethanol for 5 min and treated in activation medium containing CB and DMAP for 6 h,and (15.07±5.53)% of parthenotes developed to blastocyst stage,higher(P0.05) than the other combined treatments.

Key concepts: Blastocyst, Cytochalasin B, Ethanol, Cycloheximide, Andrology, Cleavage (geology), Parthenogenesis, Oocyte activation

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Effects of ethanol activation on the rates of cleavage and blastocyst of porcine IVM oocytes — Research Paper | ScholarLens