Study on in Vitro Maturation of Porcine Oocytes and Ethanol Activation

The objective of the present study was to investigate the effect of two different basic in vitro maturation(IVM) mediums(mTCM-199,NCSU23),concentration and treatment time of pregnant mare serum gonadotropin(PMSG) and human chorionic gonadotropin(HCG) on IVM of porcine oocytes;furthermore,to examine the effect of ethanol and ethanol plus various chemical agents on parthenogenetic activation and the development of parthenogenetic embryos.The rational IVM maturation of porcine oocytes system is NCSU23+20 IU/mL PMSG+20IU/mL HCG±10%PFF,of which the maturation rate was 70.3%±3.9%.The best parthenogenetic activation concentration of ethanol was 8%(activation rate : 29.6%±2.5%).The best activation length of exposure by 8% ethanol was 10 min(activation rate: 32.6%±4.1%).Activation by ethanol associated with CB+CHX got the highest activation rate(60.8%±3.2%),and the rate of cleavage,3~4 cells,6~8cells were 45.1%±3.0%,28.6%±3.7%,8.7%±1.2%,respectively.Treated by ethanol associated with CB+6-DMAP,the rates of activation,cleavage,3~4 cells,6~8cells were 51.7±1.9%,41.7±3.6%,30.3±4.3%,9.9±1.8%,respectively;although the rates of activation and cleavage were lower than that of ethanol plus CB+CHX,the rates of 3~4 cells,6~8 cells were higher.These results indicate that the best method of activation of pig oocytes by ethanol plus various chemical agents was 8% ethanol treating for 10 min,and then oocytes treated by CB+6-DAMP for another 7 h.