Comparison of different treatments for parthenogenetic activation of porcine oocytes matured in vitro

The systematical analysis on the effect of parthenogenetic activation protocols in vitro maturation of porcine oocytes was carried out in the present study.Comparison of parthenogenetic development of porcine oocytes treated by ethanol(EH),ionomycin(Ion:5 μmol/L),strontium chloride hexahydrate(Sr2+:10 mmol/L),6-dimethylaminopurine(6-DMAP:2 mmol/L)and cycloheximide(CHX:10 mg/L) activation treatments.The results showed that(1)The treatment of concentrations of 9%EH in the NCSU-23 medium and exposure times of 10 min for the in vitro matured porcine oocytes was better than 9%EH in the NCSU-23 medium and exposure times of 15 min.(2)Oocytes were activated with the NCSU-23 medium containing 9% ethanol for 10 min.After that,Oocytes were incubated in the NCSU-23 medium supplemented with CHX,6-DMAP,Sr2+,CHX+Sr2+、Sr2++6-DMAP,CHX+6-DMAP and CHX+6-DMAP+Sr2+ for 3-4 h respectively,the EH+6-DMAP treatment was better,the rate of cleavage and the rate of blastocyst formation were 82.86% and 22.86% respectively.(3)Chemical activation(Ion+6-DMAP and 9%EH for 10 min+6-DMAP)and electric activation(50 V/mm,50 μs,2 t)of porcine oocytes were explored in this study,the development effect of the porcine parthenogenetic embryos after ionomycin activation was better and the rate of blastocyst of Ion+6-DMAP treatment was 37.50%.(4)The effect of different quality of porcine cumulus oocyte complexes(COCs) was studied on parthenogenesis development after parthenogenetic activation.The rate of cleavage and blastocysts in the group of 4-6 layers of cumulus cells and the group of 6 layers of cumulus cells were(68.99%,32.56%) and(75.36%,37.68%) respectively and they were not significantly(P0.05),but these two groups were significantly higher(P0.05) and were more efficient than other groups.Therefore,cells of the two groups were the optimal research material of experiment for parthenogenetic activation in vitro embryo development in porcine.