Cloning and sequence analysis of Calcium-sensing receptorgene cDNA
Guo Shu-hua
Abstract
Guo Shu-hua
Abstract
By RT-PCR technology,423bpCaR gene cDNA was amplified from Pregnancy Rat Uterus on nine day and cloned in T site of PGEM-T Vector.The sequencing results and sequence analysis showed that Calcium-sensing receptor gene cDNA sequence was succeed in amplifying and cloning.
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By RT-PCR technology,423bpCaR gene cDNA was amplified from Pregnancy Rat Uterus on nine day and cloned in T site of PGEM-T Vector.The sequencing results and sequence analysis showed that Calcium-sensing receptor gene cDNA sequence was succeed in amplifying and cloning.
Key concepts: Complementary DNA, Cloning (programming), Sequence analysis, Biology, Gene, Molecular biology, Sequence (biology), Molecular cloning