Cloning and Sequencing of Mouse Adiponectin Receptor 2 Gene Encoding Region

To construct the cDNA clone and analyze the sequence of mouse adiponectin receptor 2 gene (mAdipoR2), and make a base for further research on expression and biological activities. The full mouse adipoR2 cDNA fragment was amplified by RT-PCR from mouse liver. The obtained fragment was cloned into pGEM-T vector, transformed to E.coli JM109 and identified by endonuclease digestion and sequencing. The cloned mAdipoR2 cDNA sequence was same as the reported on GenBank. mAdipoR2 protein share 91% and 95% identity with human's and rat's, respectively.