Cloning and sequencing of t-PA cDNA gene

This paper studied the cloning and sequencing of t-PA cDNA.The gene of coding t-PA was amplified by the PCR technique,using Trichoderma reesei chromosome DNA as a template.The products of PCR were inserted into pMD18-T-Vector,and transfered into E.coli DH5α.The recombinant strains were selected by ampicillin resistivity and blue-white reaction.A positive strain cloned t-PA cDNA was identified by restriction enzyme analysis and PCR technique.The cloned t-PA cDNA was sequenced,and the result showed that the cloned t-PA cDNA sequence homology was 99.81% with the t-PA cDNA published.