Protection of gypencsides and relationship with TNF-α in alcoholic fatty liver of rats

Objective To establish the rats model with alcoholic liver disease,and study the anti-apoptotic role and mechanism of gypencsides in alcoholic liver disease of rats.Methods 40 SD male rats were randomly divided into normal control group(10),model group(15) and drug intervention groups(15).Model group: ethanol lavage method was used to form alcoholic liver disease model in rats.Rats in intervention group were lavaged with gypencsides of 200 mg / kg at the same time,once a day.After 12 weeks,all rats were killed and the following items were detected using ELISA kits: serum transaminase indicators(ALT,AST),changes in lipid(TC,TG,) and TNF-α,part of the liver tissue was selected for HE staining,and the change of liver histopathology was observed under optical microscope.Part of the liver was taken to make liver homogenate and flow cytometry instrument was used to detect liver cell apoptosis.Results The model group was successfully built,and indicators changed after Gynostemma intervention.The intervention group,serum ALT,AST,TC,TG levels,as compared with the model group,decreased significantly(P 0.05).TNF-α levels,as compared with the model group,significantly decreased(P 0.05).Pathological score of the intervention group was 0.51 ± 0.49,significantly lower than that of the model group(P 0.05).Apoptosis rate of model group was obviously higher than that of normal group(P 0.05),the apoptosis rate of intervention group was obviously lower than that of the model group(P 0.05).Results suggested that gypencsides could significantly reduce histological damage of liver,especially played an important role in resisting apoptosis.Conclusions Gypencsides in rats of alcoholic liver disease liver had better protective effect.Results suggested that gypencsides could significantly reduce histological damage of liver,and played an important role in resisting apoptosis,the mechanism might be realized through lowering cytokine TNF-α level.