COX-2,TNF-α Expression in Rats with Chronic Alcoholic Liver Injury and the Role of Royal Jelly Intervention

Objective To observe expression of COX-2 and TNF-αin rats with alcoholic liver injury and the influence of royal jelly on its expression.Methods Forty SD rat9 were randomly divided into 3 groups.The control group(15 rats) received gastric infusion with normal saline,the model group(15 rats) were challenged by gastric infusion with red star Erguotou(56%),corn oil and pyrazole,and intervention group(10 rats) received royal jelly 1.0g/(kg.d) besides the same treatment as in the model group.The experiment lasted for 12 weeks and all the rats were sacrificed at the end of the experiment to get specimens.Serum aspartate transaminase(AST),alanine aminotransferase(ALT),albumin(A),and so on were determined by automatic biochemical analyzer.HE staining of liver was used to detect histopathological changes. SABC immunohistochemical assay was used to survey COX-2 and TNF-αexpression and their quantitative analysis were performed by Olympus DP70 microscopy imaging system and Image-prodiscovery5.1 image analysis system.Results The model group had obvious hepatocellular injury and the intervention group improved to some extent.The model group had a higer level of serum AST,ALT and A than the control group(P0.05) did.Compared with the model group,the intervention group treated with royal jelly had a lower level of serum ALT and AST and higher level of serum A(P0. 05).Immunohistochemistry and image analysis have shown that liver tissue COX-2 and TNF-αexpression in the model group were significantly higher than those in the control group(P0.05),and liver tissue COX-2 and TNF-αexpression in intervention group were lower than those in model group(P0.05 ).Conclusion The royal jelly has some certain protective effects on alcohol-induced liver injury for its inhibitory action of COX-2 and TNF-αexpression during the formation of alcoholic liver disease.