2010•Unpublished venueRequires access

Determination the Content of Oleanolic acid in Ganjian capsule by HPLC

Pumc Pharmaceutical

Abstract

Objective To establish the HPLC method for determining the content of Oleanolic acid in Ganjian Capsule.Method The column was Kromasil C-18(250mm×4.6mm,5um) The mobile phase was acetonitrile-water(9:1) the detection wavelength was 210nm the column temperature was 30℃ with the flow rate 0f 1.0ml/min.Results The linear range was 40.16 to 200.8ug/ml for paeonol(r= 0.9998 The recovery was 96.49% and with RSD =0.68% Conclusion The method is accurate and highly reproducible.It can be a method for quality control of Ganjian Capsule.

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Key concepts: Chromatography, Oleanolic acid, Capsule, High-performance liquid chromatography, Paeonol, Content determination, Chemistry, Linear range