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2011•Medical Journal of the Chinese People's Armed Police ForcesRequires access

Determination of oleanolic acid in Bushenqiangshen capsule by RP - HPLC

Meng Ting

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Abstract

Objective To establish a method for determination of oleanolic acid in Bushenqiangshen capsule by RP - HPLC. Methods The determination was carried out with C18 column(250 mm×4.6 mm×5μm),Using acetonitrile -0.5%acetic(85: 15) as the mobile phase and detected at the wavelength of 210 nm.(flow rate:1 ml/min) Results Oleanolic acid showed a good linear relationship at the range of 0.05～1.00μg,r=0.9997.The average recovery was 99.80%and RSD was 0.73%.Conclusions The method was simple and quick,and can be used to determine the content of oleanolic acid in Bushenqiangshen capsule.

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Objective To establish a method for determination of oleanolic acid in Bushenqiangshen capsule by RP - HPLC. Methods The determination was carried out with C18 column(250 mm×4.6 mm×5μm),Using acetonitrile -0.5%acetic(85: 15) as the mobile phase and detected at the wavelength of 210 nm.(flow rate:1 ml/min) Results Oleanolic acid showed a good linear relationship at the range of 0.05～1.00μg,r=0.9997.The average recovery was 99.80%and RSD was 0.73%.Conclusions The method was simple and quick,and can be used to determine the content of oleanolic acid in Bushenqiangshen capsule.

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Available abstract

Objective To establish a method for determination of oleanolic acid in Bushenqiangshen capsule by RP - HPLC. Methods The determination was carried out with C18 column(250 mm×4.6 mm×5μm),Using acetonitrile -0.5%acetic(85: 15) as the mobile phase and detected at the wavelength of 210 nm.(flow rate:1 ml/min) Results Oleanolic acid showed a good linear relationship at the range of 0.05～1.00μg,r=0.9997.The average recovery was 99.80%and RSD was 0.73%.Conclusions The method was simple and quick,and can be used to determine the content of oleanolic acid in Bushenqiangshen capsule.

Key concepts: Oleanolic acid, Capsule, Chromatography, High-performance liquid chromatography, Acetic acid, Chemistry, Content determination, Acetonitrile

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