2006•Qilu Pharmaceutical AffairsRequires access

Determination of baicalin in Jierexiaoyan Capsule by HPLC

Ming Liu

Abstract

OBJECTIVE To establish an HPLC method for determination of baicalin in Jierexiaoyan capsule.METHODS The chromatographic conditions were shim-pack CLC-ODS C_(18) column(6.0×150mm),mobile phase of methanol-0.4% phosphoric acid(55∶45),flow rate of 0.90ml·min~(-1),column temperature of 40℃ and detection wavelength was set at 280nm.RESULTS The regression equation was Y=61824X+75898,then the standard curve was linear over the range of 19.7～98.5μg·ml~(-1).The average recovery was 100.3% with RSD=1.87%(n=5).CONCLUSION The HPLC method was sensitive,rapid and accurate.It can be used to control the quality of Jierexiaoyan Capsule.

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Available abstract

Key concepts: Baicalin, Chromatography, Capsule, Phosphoric acid, High-performance liquid chromatography, Chemistry, Linear range, Analytical Chemistry (journal)