Expression analysis of TLR1 gene in different tissues of Maiwa yak

【Objective】The study was designed to establish a real-time PCR assay for detection of TLR1 gene,and to further analyze the expression pattern in tissues of yaks.【Method】According to the sequence of yak TLR1 gene,the real-time PCR primers were designed,the real-time PCR was established,and theβ-actin gene was chosen to normalize the expression level of TLR1 gene.The established detection method was used to analyze the expression pattern of TLR1 gene in different tissues of yak,including heart,liver,spleen,lung,kidney,large intestine,small intestine,stomach,mammary gland,muscle and ovary.【Result】The electrophoresis results showed that both TLR1 gene andβ-actin gene included a signal band.The melting curve of target product showed that the product was specific to a single peak,suggesting the specificity of primers.Moreover,real-time quantitative PCR assay was established and used to detect the relative expression level of TLR1 gene in different tissues,and the gene expression level in small intestine was selected as a baseline.The quantitative results showed that TLR1 gene was expressed at eleven tissues examined.TLR1 gene had high expression levels in kidney,liver,spleen,lung,ovary,small intestine and low levels in stomach,mammary gland,heart,large intestine and muscle.【Conclusion】There were differences on the transcription levels of TLR1 mRNA in different tissues,which may be associated with the ability to recognize and defend pathogens for different tissues.