Determination of ginsenoside Rg_1 and ginsenoside Rb_1 in Tianqitongjing Capsule by HPLC

AIM: To establish for the content determination of ginsenoside Rg 1 and ginsenoside Rb 1 in Tianqitongjing Capsule (Radix Notoginsing, Excrementum Trogopteri seu Pteromydis, etc.). METHODS: A spherisorb C 18 column (4.6 mm×250 mm, 5 μm) was used as the analytical column and acetonitrile water as the mobile phase. The detection wavelength was at 203 nm. The flow rate was 1.0 mL·min -1 . RESULTS: The calibration curves of ginsenoside Rg 1 and ginsenoside Rb 1 were linear in the range 0.492 4.92 μg ( r =0.9999) and 0.477 4.77 μg ( r =0.9998) respectively. The average recoveries were 98.8% ( RSD =0.37%, n =6) for ginsenoside Rg 1 and 98.8% ( RSD =0.38%, n =6) for ginsenoside Rb 1. CONCLUSION: The method is simple, rapid and accurate, and the result is reliable.