Experimental study on exnotransplantation of microencapsulated olfactory bulb combined with L-NAME for the treatment of spinal cord injury

Objective To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with L-NAME in rats at the early stages of spinal cord injury.Methods Healthy SD rats were divided into three groups as following: injury control group,microencapsulated transplantation of rabbit olfactory bulb cells group,microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME group,the later two groups were quantified at 1,3,7,14,and 28 d post spinal cord injury.Spinal Evans blue content was examined with the ultraviolet spectrophotometer;Spinal cord water content was detected by caculating dry/wet weight.The change of MMP-9 expression after spinal cord injury was determined by immunohistochemical method.BBB score was designed to assess its behavioral ability.Results Evans blue content and expression of MMP-9 were significantly were significantly increased,vasogenic spinal cord edema,and loss of motor function in rats after spinal cord injury.Permeability of blood-spinal barrier and vasogenic spinal cord edema,MMP-9 were decreased,motor function was recoveried in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME.Conclusion Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in some extent.There may be a synergistic effect between olfactory ensheating cells and L-NAME.