2007•Acta Academiae Medicinae JiangxiRequires access
Effect of Microencapsulated Olfactory Bulb Xenotransplantation on Cellural Apoptosis and Functional Recovery after Spinal Cord Injury in Rats
Wang Xiang-dong
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Abstract
Objective To observe the effect of microencapsulated rabbit olfactory bulb cells xenotransplantation on cellural apoptosis and functional recovery after spinal cord transection in rats.Methods Transected injury at the T10 level of the spinal cord was performed on Sprage Dawley (SD) rats and the microcapsules, which were absorbed into gelfoams(absorbable gelatin sponge),were grafted into the lesion site. All the rats were randomly divided into 4 groups: A-normal control group; B-injury control group; C-cell grafted group and D-microcapsule grafted group(rats were transplanted with gelfoams which have absorbed microcapsulated rabbit olfactory bulb cells).Before each rat was sacrificed at 12 hours and 1、3、7、21 days after injury , BBB scale was designed to assess its behavioral ability.Then,we assessed its morphologic change with the methods of the terminal deoxynucleotidal transferase-mediated DUTP-biotinnick end labeling(TUNEL).Results Few TUNEL-positive cells were noted in group A; TUNEL method showed that group B had a large number of apoptosis cells.The statistics showed that there were two apoptosis peaks,the first one appeared 1d after injury,the second peak appeared 7d after injury.The apoptosis cells of group D were lower than that of group B(P0.01)and group C (P0.05).Meanwhile, the BBB score of group D were higher than that of groups B(P0.01)and C (P0.05),but not reached the normal level.Conclusion Apoptosis is the main reason for the secondary affection.The rabbit olfactory bulb tissue cells transplantation can inhibit the apoptosis after SCI and improve the BBB score on SD rats with SCI(P0.05). Microencapsulated olfactory bulb tissue cells transplantation have an advantage over the rabbit olfactory bulb tissue cells transplantation on SD rats with SCI(P0.05). Perhaps the inhibition of demyelination due to oligodendrocytes apoptosis that plays a great role in the secondary injury after SCI in white matter.