Effects of exnotransplantation of microencapsulated olfactory bulb combined with β-aescinate sodium on blood-spinal barrier function and vasogenic spinal cord edema in spinal cord injury rats

Objective To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with β-aescinate sodium in rats at the early stages of spinal cord injury.Methods Healthy SD rats were divided into three groups as following: injury control group,microencapsulated transplantation of rabbit olfactory bulb cells group,microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium group,the later two groups were quantified at 1,3,7,14,and 28 d post spinal cord injury.Spinal Evans blue content was examined with the ultraviolet spectrophotometer;Spinal cord water content was detected by caculating dry/wet weight.The ultrastructural change of blood-spinal barrier was observed by transmission electron microscope.BBB score was designed to assess its behavioral ability.Results Evans blue content were significantly increased,vasogenic spinal cord edema,and loss of motor function in rats after spinal cord injury.Permeability of blood-spinal barrier and vasogenic spinal cord edema were obviously decreased,motor function was recoveried in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium.Conclusion Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in some extent.There may be a synergistic effect between olfactory ensheating cells and β-aescinate sodium.