Repair of spinal cord injury by transplantation of olfactory bulb tissue block or cell suspension in rat

Objective To investigate the effect and possibility of spinal cord injury repair by transplantation of olfactory bulb tissue block or cell suspension in rats.Methods Wistar rats with T10 spinal cord hemisection were divided into 3 groups:tissue block group,cell suspension and control group,subjected to transplantion of olfactory bulb tissue block (TB),olfactory bulb cell suspension (CS) and DMEM respectively.The functional recovery of spinal cord injury was observed with combined behavioral scores at different phases.The tissue sections were made at 2 weeks and 10 weeks postoperatively to observe the axons regeneration and the survival of olfactory ensheathing cells.Results The function of the rats treated with TB or CS was significantly improved as compared with control group (P0.05).Histology revealed that both TB group and CS group had regeneration of axons repaired by olfactory ensheathing cells.Conclusion The method of treating spinal cord injury by transplanted TB or CS not only simplified operation but also had satisfactory curative effect.