An Experimental Study of Traditional Chinese Medicine Kangfuxin Inducing Apoptosis In Vitro of Peptic Carcinoma Cell Line BGC-823

Objective Investigate the apoptosis-inducing activity of Chinese medicine Kangfuxin(KFX)on human peptic carcinoma cell line BGC-823 and its related mechanism.Methods Cell proliferation in different concentration and time was determined by MTT(Methy thiazolyl tetrazolium) assay.The fluorescence flow cytometry(FCM) DNA assay and TUNEL(Terminal deoxynuxleotidel transferase mediated uridine nucleotide end labeling) were applied to detecting the changes of apoptotic rate at the early and the late apoptosis process,cell proliferation and alteration of cell cycle phase.Results After incubation of BGC-823 cells with different concentration of Kangfuxin for 24,48 and 72 hours,the IC50 were(17.85±1.06),(13.76±0.57)and(11.32±0.14)mg/mL(P0.01).Kangfuxin significantly inhibited the proliferation of BGC-823 cell and the inhibited effect was dose-and-time-dependent.FCM assay indicated that most of the cells were arrested in G_2-M and the apoptotic peak appeared.During the prolonged incubation time,the apoptosis rate was increased.At the same time,the number of S phase cells decreased.The result of TUNEL indicates that there are apoptosis and necrosis.Conclusion Kangfuxin can induce BGC-823 apoptosis in vitro.