Effect of dihydroarteannuin on the proliferation of squamous carcinoma cell line YTMLC in vitro

Objective:To observe the effect of Dihydroarteannuin on the proliferation of human squamous carcinoma cell line YTMLC in vitro and study the mechanism of Dihydroarteannuin in killing Carcinoma Cells. Methods:Cell proliferation in different concentration and time was determined by MTT(Methy thiazolyl tetrazolium) assay. The fluorescence flow cytometry (FCM) DNA assay and TUNEL (Terminal deoxynuxleotidel transferase mediated uridine nucleotide end labeling) were applied to detect the changes of apoptotic rate at early and late apoptosis process and cell proliferation and alteration of cell cycle phase. Results:After incubation of YTMLC cells with different concentration Dihydroarteannuin for 48 hours and 72 hours,the IC_ 50 were 310.456μg/ml、77.983 μg/ml (P0.01) . Dihydroarteannuin significantly inhibited the proliferation of YTMLC cell, the inhibited effect was dose-and-time-dependent. FCM assay indicated that most of the cells were arrested in G_ 0 /G_ 1 phase and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis. Conclusion:Dihydroarteannuin inhibited proliferation and induced apoptosis in the human lung squamous carcinoma cell line YTMLC in vitro.