Effects of Tanshinone II A on Proliferation and apoptosis of the Human Gastric Cancer Cell Line BGC-823

BACKGROUND AND AIM:Effects of tanshinoneⅡA(TanⅡA)on proliferation and apoptosis of the human gastric cancer cell line BGC-823 were studied.MATERIALS AND METHODS:The inhibitory effect on proliferation of BGC-823 cells was evaluated in vitro by using MTT colorimetric assay after cells were treated with TanⅡA at various concentrations(0-10 μg/ml)for 48 h and 72 h.Cell apoptosis was analyzed by HE staining,DNA agarose gel electrophoresis and flow cytometry after BGC-823 cells were treated with 2,5,10 μg/ml TanⅡA for 72 h.RESULTS:TanⅡA inhibited the proliferation of BGC-823 cell markedly.The typical morphological features indicative of apoptotic state were seen under the light microscope.The DNA ladder was generated after treatment with TanⅡA at the concentrations of 5 and 10 μg/ml.The apoptotic rates were(20.60±1.84)% and(31.03±1.47)% in BGC-823 cells treated with 5,10 μg/ml of TanⅡA,respectively.There was marked difference compared with the control group whose apoptotic rate was(5.23±0.39)%.CONCLUSION:TanⅡA could inhibit proliferation and induce apoptosis of human gastric cancer cell line BGC-823 in this test.